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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Resistance to excision determines efficiency of hepatitis C virus RNA-dependent RNA polymerase inhibition by nucleotide analogs
doi: 10.1074/jbc.RA120.013422
Figure Lengend Snippet: Incorporation of CTP and CTP analogs during elongation. The time courses for incorporation of CTP (2, 5, 15, 50, 100, and 200 μm) (A), 2′C-Me-CTP (1, 2.5, 5, 10, 50, and 100 μm) (B), 2′C-Me-2′F-CTP (1, 2.5, 5, 10, 50, and 100 μm) (C), and 4′-azido-CTP (1, 2.5, 5, 10, 50, and 100 μm) (D) were collected using rapid quenched flow. The data were fit using KinTek Explorer using Scheme 1. The solid line represents the best fit generated during data fitting. The results from the fit are summarized in Table 1.
Article Snippet: Conflict of interest — K. A. J. is president of
Techniques: Generated
Journal: The Journal of Biological Chemistry
Article Title: Resistance to excision determines efficiency of hepatitis C virus RNA-dependent RNA polymerase inhibition by nucleotide analogs
doi: 10.1074/jbc.RA120.013422
Figure Lengend Snippet: Incorporation of UTP and 2′C-Me-2′F-UTP. The plots show the incorporation of UTP (10, 25, 100, 200, and 400 μm) (A) and 2′C-Me-2′F-UTP (12.3, 37, 111, 333, and 1000 μm) (B). UTP incorporation was measured using rapid quenched flow, and incorporation of 2′C-Me-2′F-UTP was measured using hand quench methods. The data were fit using Scheme 1 using KinTek Explorer, and the solid line shows the best fit generated during data fitting. Results are summarized in Table 1.
Article Snippet: Conflict of interest — K. A. J. is president of
Techniques: Generated
Journal: The Journal of Biological Chemistry
Article Title: Resistance to excision determines efficiency of hepatitis C virus RNA-dependent RNA polymerase inhibition by nucleotide analogs
doi: 10.1074/jbc.RA120.013422
Figure Lengend Snippet: Pyrophosphorolysis of CMP and 2′C Modified CTP analogues. The plots show the pyrophosphorolysis of CMP (31.25, 62.5, 125, 500, and 1000 μm) (A), 2′C-Me-CMP (15.6, 31.3, 62.5, 125, 250, and 500 μm) (B) and 2′C-Me-2′F-CMP (15.6, 31.3, 62.5, 125, 250, and 500 μm) (C). The data were fit using Scheme 2 using KinTek Explorer. Solid lines represent best fit resulting from the fitting process. Results are summarized in Table 2.
Article Snippet: Conflict of interest — K. A. J. is president of
Techniques: Modification, Analogues
Journal: The Journal of Biological Chemistry
Article Title: Resistance to excision determines efficiency of hepatitis C virus RNA-dependent RNA polymerase inhibition by nucleotide analogs
doi: 10.1074/jbc.RA120.013422
Figure Lengend Snippet: Excision of UTP. The plots show UMP excision by pyrophosphorolysis (250, 500, 1000, and 2000 μm) (A) and ATP-mediated excision (1, 2, 4, and 8 mm) (B). The results were fit to Schemes 2 and and3,3, respectively, using KinTek Explorer. The solid lines represent the best fit generated during fitting. Results are summarized in Table 2.
Article Snippet: Conflict of interest — K. A. J. is president of
Techniques: Generated
Journal: The Journal of Biological Chemistry
Article Title: Resistance to excision determines efficiency of hepatitis C virus RNA-dependent RNA polymerase inhibition by nucleotide analogs
doi: 10.1074/jbc.RA120.013422
Figure Lengend Snippet: ATP-mediated excision of 2′C-modified CTP analogues. These plots show the ATP-mediated excision of CMP (A), 2′C-Me-CMP (B), and 2′C-Me-2′F-CMP (C). ATP concentrations used were 0.25, 0.5, 1, 2, 4, and 8 mm. The data were fit using Scheme 3 using KinTek Explorer. The solid lines show the best fit generated during the fitting process. Results are summarized in Table 2.
Article Snippet: Conflict of interest — K. A. J. is president of
Techniques: Modification, Analogues, Generated